Alcohol Dehydrogenase
Product name: Alcohol Dehydrogenase
CAS Number: 9031-72-5
EC Number: 1.1.1.1
Introduction
| biological source | Saccharomyces cerevisiae |
| form | lyophilized powder (contains buffer salts) |
| specific activity | ≥300 units/mg protein |
| mol wt | Mw 141-151 kDa |
| purified by | crystallization |
| storage condition | (Keep container tightly closed in a dry and well-ventilated place.) |
| sustainability | Greener Alternative Product |
| color | white to light yellow-brown, Light brown |
| optimum pH | 8.6-9.0 |
| solubility | H2O: soluble 1.0 mg/mL, clear to slightly hazy, colorless to faintly yellow soluble |
General description
Research area: Neuroscience
Yeast alcohol dehydrogenase 1 (ADH1) belongs to the family of zinc-containing alcohol dehydrogenases. It is a homotetramer with each subunit containing one catalytic domain and coenzyme-binding domain.
Application
Alcohol dehydrogenase has been used along with lactic dehydrogenase for the enzymatic reduction of acetaldehyde using sodium(R,S)-[2-3H] lactate. It has also been used to study the inhibitory effect of zinc-chelated silymarin flavonolignans on yeast alcohol dehydrogenase.
Ethanol concentration can be determined colorimentrically by monitoring the enzymatic reduction of NAD using alcohol dehydrogenase after preremoval of the aldehyde group.