Ascorbate Oxidase
Product name: Ascorbate Oxidase
Synonym(s): L-Ascorbate:oxygen oxidoreductase, Ascorbase
CAS Number: 9029-44-1
Introduction
| biological source | plant (Cucurbita spp.) |
| Quality Level | 200 |
| form | lyophilized powder |
| specific activity | 1,000-3,000 units/mg protein |
| storage temp. | 2-8°C |
General description
Ascorbate oxidase is a homodimeric enzyme, which comprises 552 amino acid residues per subunit (zucchini). It corresponds to a molecular mass of 70kDa per subunit. This enzyme is mainly found in plants, fungi and eubacteria.
Biochem/physiol Actions
Ascorbate oxidase (AO) participates in cell growth by regulating reduction/oxidation (redox) of the apoplast. This enzyme can synthesize the oxidative molecule dehydroascorbate acid (DHA) in the apoplast. It is also involved in cell elongation and enlargement development.
Ascorbate oxidase converts ascorbic acid to dehydroascorbic acid. It is highly specific for L-ascorbic acid and a few analogs. Ascorbate oxidase exists as a dimer and has a molecular weight of approximately 140 kDa.
Unit Definition
One unit will oxidize 1.0 μmole of L-ascorbate to dehydroascorbate per min at pH 5.6 at 25 °C.
Physical form
Lyophilized powder containing buffers and sucrose as stabilizer.
