5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside
Product name:5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside
Synonym(s): 5-Bromo-4-chloro-3-indolyl β-D-galactoside, BCIG, X-Gal
CAS Number: 7240-90-6
Introduction
| Product Name | 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside, ≥98%, powder |
| Quality Level | 200 |
| assay | ≥98% |
| form | powder |
| solubility | DMF: methanol (1:1): 50 mg/mL |
| Storage temp. | −20°C |
| SMILES string | OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)c(Cl)c23)[C@H](O)[C@@H](O)[C@H]1O |
| InChI | 1S/C14H15BrClNO6/c15-5-1-2-6-9(10(5)16)7(3-17-6)22-14-13(21)12(20)11(19)8(4-18)23-14/h1-3,8,11-14,17-21H,4H2/t8-,11+,12+,13-,14-/m1/s1 |
| InChI key | OPIFSICVWOWJMJ-AEOCFKNESA-N |
Application
X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.
