5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
Product name: 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
Synonym(s): 5-Bromo-4-chloro-3-indoxyl phosphate p-toluidine salt, BCIP® p-toluidine salt, X-phosphate p-toluidine salt
CAS Number: 6578-06-9
Introduction
| Product Name | 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt (BCIP) |
| Items | Specifications |
| Appearance | White to light yellow powder |
| Solubility (0.1% DMF) | Colorless to light yellow solution |
| Purity | ≥98.0% |
| Moisture (KF) | ≤5.0% |
General description
5-bromo-4-chloro-3-indolyl phosphate (BCIP) is an histochemical phosphatase stain.
Application
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt has been used in in situ hybridization.
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
For the colorimetric detection of alkaline phosphatase-labeled molecules.
Biochem/physiol Actions
5-bromo-4-chloro-3-indolyl phosphate (BCIP) can be used to visualize the phosphatase activity.[1] It can be used as a substrate to monitor secreted embryonic alkaline phosphatase activity in solution.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Substrates
Histochemical substrate for alkaline phosphatase.
