5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
Product name: 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
Synonym(s): 5-Bromo-4-chloro-3-indoxyl phosphate p-toluidine salt, BCIP® p-toluidine salt, X-phosphate p-toluidine salt
CAS Number: 6578-06-9
Introduction
| Product Name | 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt, ≥99% (HPLC) |
| Quality Level | 200 |
| assay | ≥99% (HPLC) |
| form | powder |
| solubility | DMF: 20 mg/mL, clear to slightly hazy, yellow |
| storage temp. | −20°C |
| SMILES string | Cc1ccc(N)cc1.OP(O)(=O)Oc2c[nH]c3ccc(Br)c(Cl)c23 |
| InChI | 1S/C8H6BrClNO4P.C7H9N/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;1-6-2-4-7(8)5-3-6/h1-3,11H,(H2,12,13,14);2-5H,8H2,1H3 |
| InChI key | QEIFSLUFHRCVQL-UHFFFAOYSA-N |
General description
5-bromo-4-chloro-3-indolyl phosphate (BCIP) is an histochemical phosphatase stain.
Application
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt has been used in in situ hybridization.
For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.
BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.
For the colorimetric detection of alkaline phosphatase-labeled molecules.
Biochem/physiol Actions
5-bromo-4-chloro-3-indolyl phosphate (BCIP) can be used to visualize the phosphatase activity.[1] It can be used as a substrate to monitor secreted embryonic alkaline phosphatase activity in solution.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Substrates
Histochemical substrate for alkaline phosphatase.
