p-Nitrophenyl phosphate disodium salt hexahydrate (PNPP)
Product name: p-Nitrophenyl phosphate disodium salt hexahydrate
Synonym(s): p-Nitrophenyl Phosphate, Disodium Salt, Hexahydrate, pNPP, 2Na
CAS Number: 4264-83-9
Introduction
| Product Name | p-Nitrophenyl phosphate disodium salt hexahydrate (PNPP) |
| Items | Specifications |
| Appearance | Off-White to pale yellow crystalline powder |
| Purity | ≥99.0% |
| Water content (by KF) | 29±2% |
| p-Nitrophenol | ≤0.1% |
| Free inorganic phosphates | ≤1% |
| εmax312 | ≥9800 |
| pH | 7.5-10.0 |
General description
Excellent substrate for alkaline phosphatase-based ELISA assays. Produces a yellow soluble end product that can be read at 405 nm to 410 nm. Both kinetic and endpoint determinations can be performed. ε max: ≥9600 cm-1 M-1 at 311 nm in 0.4 M Na2CO3.
Biochem/physiol Actions
Cell permeable: no
Primary Target
Substrate for alkaline phosphatase-based ELISA assays
Product does not compete with ATP.
Reversible: no
