p-Nitrophenyl phosphate disodium salt hexahydrate (PNPP)
Product name: p-Nitrophenyl phosphate disodium salt hexahydrate
Synonym(s): p-Nitrophenyl Phosphate, Disodium Salt, Hexahydrate, pNPP, 2Na
CAS Number: 4264-83-9
Introduction
| Quality Level | 100 |
| assay | ≥99% (HPLC) |
| form | crystalline solid |
| storage temp. | 2-8°C |
| storage condition | OK to freeze protect from light |
| impurities | ≤0.005% p-nitrophenol (weight) ≤1% free phosphate (weight) |
| color | light yellow |
| solubility | water: 5 mg/mL |
| shipped in | ambient |
General description
Excellent substrate for alkaline phosphatase-based ELISA assays. Produces a yellow soluble end product that can be read at 405 nm to 410 nm. Both kinetic and endpoint determinations can be performed. ε max: ≥9600 cm-1 M-1 at 311 nm in 0.4 M Na2CO3.
Biochem/physiol Actions
Cell permeable: no
Primary Target
Substrate for alkaline phosphatase-based ELISA assays
Product does not compete with ATP.
Reversible: no
