p-Nitroblue Tetrazolium Chloride
Product name: Nitrotetrazolium Blue chloride
Synonym(s): 2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride, 3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride], p-Nitro-Blue tetrazolium chloride, p-Nitrotetrazolium blue, NBT, Nitro BT
CAS Number: 298-83-9
Introduction
| description | RTECS – XF8045000 |
| assay | ≥98% (TLC) |
| form | crystalline solid |
| potency | 3-4 μM IC50 |
| storage condition | OK to freeze desiccated (hygroscopic) protect from light |
| color | yellow |
| solubility | methanol: soluble water: soluble |
General description
NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC50 = 3-4 µM). A well-known scavenger of superoxide anions. Useful as a substrate for alkaline phosphatase, often in conjunction with BCIP.
Warning
Toxicity: Harmful (C)
Reconstitution
Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage.
